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US 2026/0062747 A1Published application
Polynucleotide sequencing
AssigneeIllumina, Inc.
InventorsXiaolin Wu, Patrick McCauley
Life of this patent
Estimating the term…
Abstract
A polynucleotide sequencing method includes a wash step that employs a composition including a polymerase. The composition may also include a plurality of nucleotides. The composition may be configured to prevent the polymerase from incorporating one of the plurality of nucleotides into a copy polynucleotide strand. The composition may be substantially free of Mg2+
Claims8
A cartridge for use with a sequencing apparatus, the cartridge comprising:
- (a) a first chamber comprising an incorporation composition comprising a first plurality of reagents for incorporating a blocked, labeled nucleotide into a copy polynucleotide strand complementary to at least a portion of a template polynucleotide strand;
- (b) a second chamber comprising a detection composition for identifying the blocked, labeled nucleotide incorporated into the copy polynucleotide strand;
- (c) a third chamber comprising a cleavage composition comprising a second plurality of reagents for removing a label and a blocking moiety from the blocked, labeled nucleotide incorporated into the copy polynucleotide strand;
- and
- (d) a fourth chamber comprising a first wash composition comprising a polymerase and a plurality of nucleotides, wherein the first wash composition is configured to prevent the second polymerase from incorporating one of the plurality of nucleotides into the copy polynucleotide strand.
2 more elements
A kit for use with a sequencing apparatus, the kit comprising:
- (a) a first container containing an incorporation composition comprising a first plurality of reagents for incorporating a blocked, labeled nucleotide into a copy polynucleotide strand complementary to at least a portion of a template polynucleotide strand;
- (b) a second container comprising a detection composition for identifying the blocked, labeled nucleotide incorporated into the copy polynucleotide strand;
- (c) a third container comprising a cleavage composition comprising a second plurality of reagents for removing a label and a blocking moiety from the blocked, labeled nucleotide incorporated into the copy polynucleotide strand;
- and
- (d) a fourth container comprising a first wash composition comprising a polymerase and a plurality of nucleotides, wherein the first wash composition is configured to prevent the second polymerase from incorporating one of the plurality of nucleotides into the copy polynucleotide strand.
2 more elements
Dependencies are inferred from the claim text.