Systems and methods to improve nucleic acid synthesis and production

A method, comprising:

• receiving circular deoxyribonucleic acid (DNA) template at a nucleic acid amplification module;
• generating, via the nucleic acid amplification module, an amplified product from the circular DNA template utilizing an amplification reaction, wherein the nucleic acid amplification module comprises a pump configured to cause flow of a portion of the amplification reaction throughout a quality control panel;
• performing real time inline monitoring of the amplification reaction at the quality control panel via a plurality of sensors in communication with a controller having a memory and a processor, wherein the controller is configured to receive feedback from the plurality of sensors;
• and
• ceasing, in response to control signals from the controller, the amplification reaction and providing the amplified product to a purification module upon reaching a defined quality range and generating a purified nucleic acid product.

A non-transitory computer-readable medium, the computer-readable medium comprising processor-executable code that, when executed by a processing system, causes the processing system to:

• provide control signals to a nucleic acid amplification module to generate an amplified product from circular deoxyribonucleic acid (DNA) template utilizing a rolling circle amplification reaction under isothermal conditions;
• receive feedback from a plurality of sensors performing real time inline measurements of kinetics and conditions of the rolling circle amplification reaction;
• and
• provide control signals based on the feedback to regulate purification of the rolling circle amplification reaction.