Methods for producing gamma-cyclodextrins
AssigneeBeren Therapeutics P.B.C.
InventorsEli GROBAN, Zhihao HU, Steven PFEIFFER, Benjamin RIZKIN, Gabor BENKOVICS
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Abstract
Provided herein are methods for the enzymatic production of gamma-cyclodextrin from sucrose. In some cases, the methods involve contacting sucrose with one or more enzymes to convert sucrose to amylose, followed by contacting the amylose with one or more enzymes to convert the amylose to gamma-cyclodextrin. In some cases, the methods produce higher yields of gamma-cyclodextrin relative to alpha-cyclodextrin, beta-cyclodextrin, or both.
Claims34
A method of producing a composition comprising cyclodextrin, the method comprising:
- (a) contacting sucrose with an enzyme, or an enzyme mixture, capable of converting sucrose to amylose under conditions that permit the conversion of the sucrose to amylose, thereby producing amylose;
- and
- (b) contacting the amylose produced in (a) with an enzyme capable of converting amylose to cyclodextrin under conditions that permit the conversion of the amylose to cyclodextrin, thereby producing the composition comprising cyclodextrin,
- wherein the composition comprising cyclodextrin comprises gamma-cyclodextrin in an amount and/or concentration greater than alpha-cyclodextrin, beta-cyclodextrin, or both.
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-36. (canceled)
The method of claim 36, wherein the enzyme or at least one enzyme of the enzyme mixture of (a), the variant enzyme of (b), or both, is provided in a cell slurry or whole cell lysate, and wherein the cell slurry or whole cell lysate further comprises an additive selected from the group consisting of
- mannitol, sorbitol, sucrose and combinations thereof.
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An enzyme comprising or consisting of an amino acid sequence of any one of SEQ ID NOs:
- 1-31, or an enzyme comprising or consisting of an amino acid sequence having at least about 70% sequence identity to the amino acid sequence of any one of SEQ ID NOs:
- 1-31.
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Dependencies are inferred from the claim text.