Modified esterase

Abstract

The present invention provides any one of the following modified esterases (i) to (iii): (i) a modified esterase comprising an amino acid sequence in which at least one amino acid at a site selected from the group consisting of (1) to (24) below in the amino acid sequence of SEQ ID NO: 1 or 3 is substituted with another amino acid, (1) V64 (2) S65 (3) A102 (4) A109 (5) L151 (6) I153 (7) Y173 (8) Y197 (9) L200 (10) I211 (11) F217 (12) S220 (13) D222 (14) D227 (15) L229 (16) L242 (17) A245 (18) V257 (19) L298 (20) T301 (21) V326 (22) S329 (23) A350 (24) T371; (ii) a modified esterase in which one or more amino acids are further substituted (except for the amino acid site substituted in (i)), added, inserted, or deleted (except for the amino acid site substituted in (i)) in the modified esterase of (i), and the reactivity to (1R,3R)-methyl 3-(2-chloro-3,3,3-trifluoroprop-1-en-1-yl)-2,2-dimethylcyclopropane-1-carboxylate is improved compared to the reactivity of an esterase consisting of the amino acid sequence of SEQ ID NO: 1 or 3; (iii) a modified esterase in which an amino acid(s) other than the amino acid(s) substituted in (i) is further substituted with another amino acid(s), wherein the modified esterase has 70% or more identity with the modified esterase of (i) and has improved reactivity to (1R,3R)-methyl 3-(2-chloro-3,3,3-trifluoroprop-1-en-1-yl)-2,2-dimethylcyclopropane-1-carboxylate compared to the reactivity of the esterase consisting of the amino acid sequence of SEQ ID NO: 1 or 3.

Dependencies are inferred from the claim text.