Systems and methods to improve nucleic acid synthesis and production

A method for purifying a deoxyribonucleic acid (DNA) product from a rolling circle amplification (RCA) reaction, comprising:

• obtaining a reaction volume having the DNA product from the RCA reaction;
• diluting the reaction volume with a buffer to form a diluted reaction volume;
• and
• flowing the diluted reaction volume through a first purification process configured to remove protein and to generate a protein-depleted DNA product.

A non-transitory computer-readable medium, the computer-readable medium comprising processor-executable code that, when executed by a processing system, causes the processing system to:

• obtain a reaction volume having a deoxyribonucleic acid (DNA) product from a rolling circle amplification reaction;
• dilute the reaction volume with a buffer to form a diluted reaction volume;
• flow the diluted reaction buffer through a first purification process configured to remove protein and to generate a protein-depleted DNA product;
• flow the protein-depleted DNA product through a second purification process configured to positively select for the DNA product using anion exchange;
• and
• elute of a purified DNA product from the second purification process.