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AssigneeTakeda Pharmaceutical Company Limited
InventorsMeinhard Hasslacher, Christian FIEDLER, Christa MAYER, Artur MITTERER
Estimating the term…
Provided herein are methods for purifying recombinant A Disintegrin-like and Metallopeptidase with Thrombospondin Type 1 Motif 13 (ADAMTS13) protein from a sample. The method comprises enriching for ADAMTS13 protein by chromatographically contacting the sample with hydroxyapatite under conditions that allow ADAMTS13 protein to appear in the eluate or supernatant from the hydroxylapatite. The methods may further comprise tandem chromatography with a mixed mode cation exchange/hydrophobic interaction resin that binds ADAMTS13 protein. Additional optional steps involve ultrafiltration/diafiltration, anion exchange chromatography, cation exchange chromatography, and viral inactivation. Also provided herein are methods for inactivating virus contaminants in protein samples, where the protein is immobilized on a support. Also provided herein are compositions of ADAMTS13 prepared according to said methods.
A method for purifying recombinant a disintegrin-like and metallopeptidase with thrombospondin type 1 motif 13 (ADAMTS13) protein from a sample comprising ADAMTS13 protein and non-ADAMTS13 impurities, the method comprising chromatographically contacting the sample with hydroxyapatite under conditions that allow said ADAMTS13 protein to appear in an eluate or a supernatant from said hydroxyapatite.
The method according to 9, wherein said virus inactivation step comprises adding a solvent-detergent mixture comprising a non-ionic detergent and an organic solvent to said ADAMTS13 protein.
A method for inactivating virus contaminants in a protein sample, said method comprising:
Dependencies are inferred from the claim text.