Selective functional enhancement of stem cells and or their genetic material, with gene transfection for short-and long-term treatment of age-related disease states

A method comprising:

• (a) administering one or more immunostimulants to a donor or a patient to mobilize stem cells into the bloodstream;
• (b) collecting and isolating mesenchymal stem cells (MSCs) from blood of the donor or patient;
• (c) generating reprogrammed stem cells (RSCs) by exposing the MSCs to one or more histone-modifying agents that alter methylation and/or acetylation patterns;
• (d) producing gene-enhanced stem cells (GESs) by transfecting the RSCs with a vector, wherein the vector comprises an adeno-associated virus (AAV), a cytomegalovirus (CMV), or a plasmid DNA vector,
• wherein the vector includes a gene which encodes human telomerase reverse transcriptase (hTERT) and/or telomerase RNA component (TERC);
• (e) culturing the GESs to express the human telomerase reverse transcriptase (hTERT) and/or telomerase RNA component (TERC);
• (f) isolating exosomes from the GESs;
• and
• (g) administering the isolated exosomes to a patient.