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US 2026/0061055 A1Published application
Methods of producing expanded natural killer cells from cryopreserved apheresis samples and uses thereof
OwnerBruno FIGUEROA, Alexandra GRELLA +1inventors
InventorsBruno FIGUEROA, Alexandra GRELLA, Sarah STURTEVANT
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Abstract
Provided herein are methods of producing expanded natural killer cells from cryopreserved blood samples, compositions comprising natural killer cells produced by the methods, and uses thereof.
Claims37
A method of producing expanded natural killer (NK) cells comprising:
- (i) thawing a cryopreserved blood sample obtained from a subject;
- (ii) obtaining peripheral blood mononuclear cells (PBMCs) from the thawed blood sample without the use of density gradient separation;
- (iii) depleting the PBMCs of CD3+ cells;
- (iv) culturing the PBMCs in a medium with membrane-bound IL-21 to expand NK cells contained in the PBMCs;
- and
- (v) harvesting the expanded NK cells when population doubling reaches a threshold limit.
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Dependencies are inferred from the claim text.