Image based autofocus of optical systems
AssigneeElement Biosciences, Inc.
InventorsYanfei JIANG, Steve Xiangling CHEN, Jordan NEYSMITH, Michael PREVITE
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Abstract
The present disclosure describes illumination methods and systems for illumination as well as methods and systems for autofocusing the systems. The systems can be used for, for example, microscopy and sequencing platforms. The methods and systems of the present disclosure can provide fast and accurate autofocusing, which can reduce error and improve system throughput.
Claims29
A method for focusing an optical system, comprising:
- (a) receiving an image of a substrate of said optical system, wherein a portion and less than all of said image is in focus, and wherein said portion of said image in focus is offset from a center of said image;
- (b) determining, using at least a distance from said portion of said image in focus and said center of said image, an amount of defocus in said image;
- and
- (c) adjusting a parameter of said optical system to adjust for said defocus.
-3. (canceled)
(canceled)
-13. (canceled)
A method of focusing an optical system, comprising:
- (a) imaging, using a detector, a substrate tilted at a tilt angle, wherein an image of said substrate comprises an in focus portion and an out-of-focus portion;
- (b) determining, using a processor, a defocus of the optical system based at least in part on said tilt angle and a distance of said in focus portion from a center of said image;
- (c) adjusting said substrate to remove said tilt angle;
- and
- (d) adjusting said substrate by said defocus, thereby focusing said optical system.
-31. (canceled)
-41. (canceled)
A method of focusing an optical system, comprising:
- (a) imaging, using a detector tilted at a tilt angle, a substrate, wherein an image of said substrate comprises an in focus portion and an out-of-focus portion;
- (b) determining, using a processor, a defocus of the optical system based at least in part on said tilt angle and a distance of said in focus portion from a center of said image;
- and
- (c) adjusting said substrate by said defocus, thereby focusing said optical system.
-52. (canceled)
Dependencies are inferred from the claim text.