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US 2026/0055364 A1Published application
Methods for Improving Sperm Function and Fertilizing Ability for Assisted Reproduction Techniques
AssigneesDario KRAPF, Consejo Nacional De Investigaciones Cientificas Y Tecnicas (Conicet), Universidad Nacional De Rosario
InventorsDario KRAPF, Mariano Gabriel BUFFONE, Guillermina Maria LUQUE, Matias GOMEZ ELIAS
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Abstract
The disclosure provides methods for improving sperm function and fertilizing ability for assisted reproduction techniques. The methods provided by the disclosure, in some embodiments entail sequential changes of temperature, bicarbonate concentration, albumin concentration and calcium ionophore.
Claims16
A method for preparing a mammalian sperm wherein the method comprises:
- (a) incubating the mammalian sperm in the presence of Ca2+ ionophore
- (b) washing the mammalian sperm of step (a) with fresh medium to remove Ca2+ ionophore
- (c) incubating the mammalian sperm of step (b) at temperature between 38° C. and 43° C. in a medium containing HCO3 and,
- (d) transferring an appropriate concentration of mammalian sperm of step (c) to an appropriate medium,
- wherein said mammalian sperm are suitable for use in the assisted reproductive techniques (ART).
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Dependencies are inferred from the claim text.