Phenotypic and biological assessment of microbes
AssigneeInscripta, Inc.
InventorsGlenn Patrick Hein, Christopher Michael Rath, Theodore M. Tarasow
Life of this patent
Estimating the term…
Abstract
The present disclosure provides technologies for predicting a phenotype of a microbial cell using machine learning models trained using high-content imaging data (HCI). Also provided are methods of engineering a microbial cell to possess a phenotype of interest. Example phenotypes include the production of a target compound or biomolecule of interest. The provided technologies are useful for the efficient biomanufacturing of target compounds.
Claims24
A method for determining or predicting a phenotype of a microbial cell, comprising:
- (a) obtaining at least one high-content image of the microbial cell;
- (b) executing a computer-based model that was trained with image features of known microbial cell phenotypes;
- and
- (c) generating, with a processor, a determination or prediction of the phenotype of the microbial cell.
6-7. (canceled)
9-26. (canceled)
A method of engineering a microbial cell to have a desired phenotype, comprising:
- (a) generating, in silico, at least one design candidate microbial cell incorporating at least one genetic feature associated with a desired phenotype;
- (b) engineering the at least one design candidate microbial cell;
- (c) culturing the at least one design candidate microbial cell;
- and
- (d) determining the phenotype of the at least one design candidate microbial cell using a high-content imaging (HCI)-based model.
30-31. (canceled)
A bioreactor or fermentation monitoring system, comprising:
- (a) a tank for culturing a population of microbial cells,
- (b) a camera for obtaining high content images of the population of microbial cells in the tank,
- (c) a processing system connected to the camera such that the high content images obtained by the camera are used to predict a phenotype or function of individual cells within the population of microbial cells while the population of cells is being cultured.
35-61. (canceled)
Dependencies are inferred from the claim text.