Compositions comprising cell-delivered vesicles and uses thereof
AssigneeProKidney
InventorsTimothy A. BERTRAM, Deepak JAIN
Life of this patent
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Abstract
Provided herein are, inter alia, extracellular products (e.g., vesicles such as microvesicles, e.g., exosomes) produced by renal cells (such as bioactive renal cells, e.g., selected renal cells). Methods of altering components (such as miRNAs or proteins) of vesicles produced by cells, as well as methods of producing vesicles comprising various compounds are also included. Also provided are diagnostic and treatment methods.
Claims16
1-102. (canceled)
A method for monitoring treatment with a bioactive renal cell population in a subject who has been administered the bioactive renal cell population, comprising:
- obtaining a vesicle from a sample of the subject;
- and
- detecting whether at least one compound is in the vesicle, wherein:
- (i) the at least one compound is a protein, and the protein is CD9, CD81, CD146, CD326, CD40, CD42a, CD44, CD49e, and/or SSEA-4:
- (ii) the at least one compound comprises miRNAs, wherein the miRNAs include at least two of miR-145, miR-22, miR-7, miR-10a, miR-143, and/or let7b;
- and/or
- (iii) the at least one compound is not expressed or produced by renal cells in a native kidney.
(canceled)
A method of detecting the level of at least one miRNA in vesicles from a bioactive renal cell population, the method comprising-
- (i) detecting whether one or more of the following miRNA molecules is increased in the vesicles compared to a control:
- miR-1248, miR-3168, miR-362-5p, miR-7113-5p, miR-758-3p, miR-937-3p, miR-4455, miR-4521, miR-203a-3p, miR-22-3p, miR-574-3p, miR-181b-5p, miR-1260b, and/or miR-181b-5p;
- and/or
- (ii) detecting whether one or more of the following miRNA molecules is decreased in the vesicles compared to a control:
- miR-1-3p, miR-1-3p, miR-143-3p, miR-150-5p, miR-509-3p, miR-653-5p, miR-204-5p, miR-192-5p, and/or miR-363-3p.
115-142. (canceled)
Dependencies are inferred from the claim text.