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US 2026/0049926 A1Published application
Systems, devices and methods for sorting moving particles
AssigneeThe Regents of the University of California
InventorsRui TANG, Zunming ZHANG, Yu-Hwa LO
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Abstract
Systems, devices and methods for sorting particles utilizing focus stacking of two-dimensional images are described. Such systems, devices and methods may further provide for particle processing and may encompass, on a microfluidic scale, sample enrichment, sample mixing, sample/particle sorting, verification of sorting and feedback-based optical sorting.
Claims26
A system for sorting a particle in a sample comprising:
- a) a flow cell configured to allow the particle to move in a flow path in a flow direction;
- b) a sorting module fluidly connected to the flow cell;
- c) an illumination module comprising a light redirection device configured to diffract or scan a light beam in a scanning direction, wherein the scanning direction is about perpendicular to the flow direction, and wherein the light beam is propagated in a light propagating direction about orthogonal to a plane comprising the flow direction and the light beam diffraction or scan direction;
- d) a detection module comprising at least one spatial filter, the at least one spatial filter comprising a forward spatial filter and the at least one spatial filter comprises:
- i. a front surface;
- ii. a plurality of apertures, the plurality of apertures arranged in a line comprising an angle with the flow direction, wherein the detection module is configured to generate a time-dependent signal upon detection of the particle within the flow path, wherein the time-dependent signal correlates to a particle feature of the particle;
- and
- e) a data processing module configured to generate a gating signal using the time-dependent signal, and
- wherein the sorting module is configured to receive the gating signal and sort the particle in response to the gating signal.
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A method for particle sorting, the method comprising:
- a) providing a sorting system comprising:
- i) a flow cell configured to allow a particle to move in a flow path in a flow direction;
- ii) a sorting module fluidly connected to the flow cell;
- iii) an illumination module comprising a light redirection device configured to diffract or scan a light beam in a diffraction or scan direction, wherein the diffraction or scan direction is about perpendicular to the flow direction, and wherein the light beam is propagated in a light propagating direction about orthogonal to a plane comprising the flow direction and the diffraction or scan direction;
- iv) a detection module comprising a spatial filter, the spatial filter comprising:
- 1. a front surface;
- 2. a plurality of apertures, the plurality of apertures arranged in a line comprising an angle with the flow direction, wherein the detection module is configured to generate a time-dependent signal upon detection of the particle within the flow path, wherein the time-dependent signal correlates to a particle feature of the particle;
- and
- v) a data processing module configured to generate a gating signal using the time-dependent signal;
- and
- b) loading a sample comprising the plurality of particles into the system;
- and
- c) initiating the system, and
- wherein, the sorting module is configured to sort the particle in response the gating signal.
11 more elements
A method for cell sorting, the method comprising:
- a) flowing a particle along a flow path through a flow cell in a flow direction;
- b) illuminating the particle via diffracting or scanning a light beam in a diffraction or scan direction, the diffraction or scan direction about perpendicular to the flow direction, the light beam propagating in a light propagating direction about orthogonal to a plane comprising the flow direction and the diffraction or scan direction;
- c) detecting the particle, wherein light having interacted with the particle transmits through an aperture of a plurality of apertures of a spatial filter, the plurality of apertures arranged in a line comprising an angle with the flow direction, wherein the detection module is configured to generate a time-dependent signal upon detection of the particle, wherein the time-dependent signal correlates to a particle feature of the particle;
- d) generating a gating signal based on aspects of the particle feature;
- and
- e) sorting the particle based on the gating signal.
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Dependencies are inferred from the claim text.