Adjuvanted immunogenic composition against neisseria meningitidis b
AssigneeSanofi Pasteur Limited
InventorsSalvador Fernando Ausar, Vinod Balhara, Anne-Gaëlle Darmont, Nausheen Rahman
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Abstract
The disclosure relates to an immunogenic composition comprising a combination of Neisseria meningitidis serogroup B antigens, said combination comprising at least one factor H binding protein (fHBP) A and at least one factor H binding protein (fHBP) B, and an aluminum hydroxyphosphate (AlPO4) adjuvant, the AlPO4 adjuvant being selected as having a point of zero charge (PZC) below 5.
Claims33
An immunogenic composition comprising a combination of Neisseria meningitidis serogroup B antigens, said combination comprising at least one factor H binding protein (fHBP) A and at least one factor H binding protein (fHBP) B, and an aluminum hydroxyphosphate (AlPO4) adjuvant, the AlPO4 adjuvant being selected as having a point of zero charge (PZC) below 5.
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A method for enhancing an immune response induced by a composition comprising a N. meningitidis fHBP B antigen against a N. meningitidis serogroup B strain expressing a fHBP B antigen heterologous to said fHBP B antigen of said composition, or
- for stabilizing at least one of fHBP A and NadA protein in an immunogenic composition, or
- for stabilizing a time of onset of sedimentation (Tonset) of a composition comprising a combination of Neisseria meningitidis serogroup B antigens, said combination comprising at least one factor H binding protein (fHBP) A and at least one factor H binding protein (fHBP) B, in a range from about 3.5 min to about 10 min, or
- for adjuvanting an immunogenic composition comprising a combination of Neisseria meningitidis serogroup B antigens, said combination comprising at least one factor H binding protein (fHBP) A and at least one factor H binding protein (fHBP) B, or
- for manufacturing an immunogenic composition comprising a combination of Neisseria meningitidis serogroup B antigens, said combination comprising at least one factor H binding protein (fHBP) A and at least one factor H binding protein (fHBP) B,
- wherein the method comprises administering an AlPO4 adjuvant having a PZC below 5 to a subject.
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A method for manufacturing an immunogenic composition comprising a combination of Neisseria meningitidis serogroup B antigens, said combination comprising at least one factor H binding protein (fHBP) A and one factor H binding protein (fHBP) B, and an AlPO4 adjuvant, the method comprising at least the steps of:
- a) selecting an AlPO4 adjuvant having a PZC below 5, and
- b) combining the AlPO4 adjuvant selected at step a) with at least one factor H binding protein (fHBP) A and at least one factor H binding protein (fHBP) B, the combination being carried out in any order.
A method for stabilizing at least one of fHBP A and NadA protein in an immunogenic composition, the method comprising at least the steps of:
- a) selecting an AlPO4 adjuvant having a PZC below 5, and
- b) combining the AlPO4 adjuvant selected at step a) with a fHBP A or NadA protein, and
- c) obtaining an immunogenic composition in which said fHBP A or NadA protein is stabilized.
A method for preparing an immunogenic composition comprising a N. meningitidis fHBP B antigen, said composition inducing an enhanced immune response against a N. meningitidis serogroup B strain expressing a fHBP B heterologous to said fHBP B antigen of said composition, the method comprising at least the steps of:
- a) selecting an AlPO4 adjuvant having a PZC below 5, and
- b) combining the AlPO4 adjuvant selected at step a) with said fHBP B antigen, and
- c) obtaining said immunogenic composition.
Dependencies are inferred from the claim text.