Magnetic bead elisa detector device and methods of use thereof

A system for detecting a target analyte in a test sample, the system comprising:

• a test sample receiving portion;
• one or more hybridization portions interconnected to the test sample receiving portion, wherein the one or more hybridization portions comprise or are capable of being loaded with:
• a first antibody coupled to a magnetic bead, the first antibody capable of specifically binding to the target analyte, and
• a second antibody coupled to an enzyme, the second antibody capable of specifically binding to the target analyte, wherein the enzyme is capable of converting a redox active molecule;
• a redox portion interconnected to the one or more hybridization chambers, wherein the redox portion comprises or is capable of being loaded with a developing solution having the redox active molecule;
• an electrode set;
• a potentiostat for performing amperometry to obtain an electrochemical measurement based on conversion of the redox active molecule;
• and
• a multitude of magnetic sources positioned for moving the magnetic bead in a sequential movement from the hybridization portion to the redox portion and optionally beyond, wherein different magnetic sources of the multitude of magnetic sources are capable of being turned on and off in a desired sequence to achieve the sequential movement of the magnetic bead.