Production of fatty alcohols in peroxisome of yarrowia lipolytica

A method for producing one or more fatty alcohols, comprising:

• (a) growing a recombinant Yarrowia lipolytica in a culture medium, wherein the recombinant Yarrowia lipolytica comprises a heterologous polynucleotide encoding a fusion protein, wherein the fusion protein comprises a first amino acid sequence at least 80% identical to the amino acid sequence of 3-ketoacyl CoA thiolase (3KAT) (SEQ ID NO:
• 1) and a second amino acid sequence at least 80% identical to the amino acid sequence of a fatty acyl-CoA reductase (FAR);
• (b) expressing the fusion protein in one or more peroxisomes in the recombinant Yarrowia lipolytica;
• and
• (c) generating one or more fatty alcohols by the recombinant Yarrowia lipolytica, whereby the one or more fatty alcohols are produced in the culture medium.