Expression cassette for target gene and use thereof
AssigneeSuzhou Heguang Kehui Biotechnology Co., Ltd
InventorsTenghui YU, Zhou YANG, Jianghong ZHANG
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Abstract
The present invention provides an expression cassette for a target gene and use thereof. Specifically, the present invention provides an expression cassette having elements such as HCR, DSE, TPL and eMlp, an encoding nucleic acid thereof, an expression vector thereof, a host cell thereof, a pharmaceutical composition thereof, a gene delivery system thereof, and use thereof. The present invention further provides a C1-INH protein-encoding nucleic acid molecule and use thereof.
Claims15
An expression cassette having a structure shown in formula I from the 5′ to 3′ end:
- wherein each “—” is independently a bond or a nucleotide linker sequence;
- Z1 is an HCR element;
- Z2 is a DSE element;
- Z3 is a TPL element;
- Z4 is an eMlp element;
- Z5 is an intron element;
- Z6 is absent or is a Kozak sequence;
- Z7 is a target gene;
- and
- Z8 is a poly(A) element.
A nucleic acid molecule encoding the serpinG1 gene, the nucleotide sequence of which has at least 87% identity, preferably at least 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% identity to the sequence as set forth in SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.