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US 2026/0035740 A1Published application
Spatially encoded biological assays
AssigneePrognosys Biosciences, Inc.
InventorMark S. Chee
Life of this patent
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Abstract
The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.
Claims20
(canceled)
A method, comprising:
- (a) providing a substrate comprising a plurality of capture areas, wherein a capture area of the plurality of capture areas comprises:
- (i) a first oligonucleotide comprising a sequence that is complementary to a nucleic acid analyte, and
- (ii) a second oligonucleotide comprising a sequence that identifies a location of the capture area on the substrate;
- (b) allowing the nucleic acid analyte to interact with the first oligonucleotide;
- and
- (c) generating a nucleic acid molecule comprising:
- (i) all or a portion of the sequence of the nucleic acid analyte, or a complement thereof and (ii) the sequence that identifies the location of the capture area, or a complement thereof.
4 more elements
Dependencies are inferred from the claim text.