Optical Determination of pH Level of Biological Samples

A method comprising:

• generating one or more images of tissue inside of a patient's mouth, wherein the one or more images depict fluorescence from the tissue;
• determining a pH level on the tissue using a model and pixel intensities of the one or more images;
• and
• generating an indication of the pH level on the tissue.

An imaging device comprising:

• a source of blue light configured to illuminate a sample;
• a source of white light configured to illuminate the sample;
• a long pass filter having a cutoff wavelength of 435 nm +/−15 nm;
• a beam splitter configured to receive light filtered by the long pass filter;
• a first band pass filter having a critical wavelength of 520 nm +/−15 nm configured to receive a first beam from the beam splitter;
• a second band pass filter having a critical wavelength of 550 nm +/−15 nm configured to receive a second beam from the beam splitter;
• a first CMOS image sensor configured to generate a first image of the first beam;
• and
• a second CMOS image sensor configured to generate a second image of the second beam.

An imaging device comprising:

• a source of blue light configured to illuminate a sample;
• a long pass filter having a cutoff wavelength of 435 nm +/−5 nm;
• a first beam splitter configured to split light filtered by the long pass filter into a first beam having wavelengths less than 470 nm +/−5 nm and an axis beam having wavelengths greater than 470 nm +/−5 nm;
• a first CMOS image sensor configured to generate a first image of the first beam;
• a second beam splitter configured to split the axis beam into a second beam having wavelengths less than 535 nm +/−5 nm and a third beam having wavelengths greater than 535 nm +/−5 nm;
• a first band pass filter having a first critical wavelength of 520 nm +/−5 nm configured to receive the second beam from the second beam splitter;
• a second band pass filter having a second critical wavelength of 550 nm +/−5 nm configured to receive the third beam from the second beam splitter;
• a second CMOS image sensor configured to generate a second image of the second beam after processing by the first band pass filter;
• and
• a third CMOS image sensor configured to generate a third image of the third beam after processing by the second band pass filter.