Prognostic and diagnostic methods for risk of acute kidney injury

A method of administering a therapeutic intervention to a subject having acute kidney injury risk comprising:

• (i) determining the subject's protein marker profile for a panel of protein markers comprising at least two protein markers selected from those set forth in Table 1;
• (ii) optionally, determining the status of at least one clinical variable or measurement for the subject, wherein the clinical variable or measurement is selected from those set forth in Table 2;
• (iii) assigning a score to the subject based on the protein marker profile in (i) and optionally the clinical variable of measurement in (ii) wherein the score is classified as positive, intermediate, and negative, said score algorithmically-derived from the normalized and mathematically transformed concentrations of protein markers in the subject's sample and optionally, the status of at least one clinical variable or measurement;
• and
• (iv) administering to the subject a therapeutic intervention based on the positive, intermediate or negative score.

A method of detecting two or more protein markers in a subject having diabetes type 2 and/or that is suspected of having acute kidney injury risk, the method comprising:

• (i) selecting a subject that has diabetes type 2 and/or that is suspected of having acute kidney injury risk;
• (ii) providing a biological sample from the subject;
• (iii) applying the biological sample to an analytical device, and
• (iv) detecting the concentration of at least two protein markers from Table 1.

A method of monitoring risk of acute kidney injury in a subject, comprising:

• (i) providing a biological sample from a subject suspected of having a risk of acute kidney injury,
• (ii) applying the biological sample to an analytical device, to
• (a) detect the concentration of at least two protein markers in the sample;
• (b) normalize said concentration of protein markers against a synthetic quantification standard and
• (c) transform the normalized protein marker concentrations;
• wherein the at least two protein markers are selected from those set forth in Table 1;
• (iii) optionally, determining the status of at least one clinical variable or measurement for the subject, wherein the clinical variable or measurement is selected from those set forth in Table 2;
• (iv) calculating a score using an algorithm based on the normalized, transformed protein markers determined in step (ii) and, optionally, the status of the clinical variable or marker determined in step (iii);
• (v) classifying the score as a positive, intermediate, or negative result;
• (vi) determining a prognosis of acute kidney injury risk in a subject as indicated by the score;
• (vii) repeating steps (i) to (vi) from the same subject at a later timepoint;
• and
• (viii) comparing the scores to determine if there is a change in acute kidney injury risk in the subject.