Linearized mrna preparation system, use thereof, and preparation method for preparing mrna by using same

Abstract

A linearized mRNA preparation system, use thereof, and a preparation method for preparing mRNA by using same. A linearization buffer system comprises: 0-150 mM Tris-Ac or phosphate, 0.5-150 mM divalent metal ions or trivalent metal ions, 0.1-0.75 U/μL restriction endonuclease, and 0-7.5 mM spermidine. The present invention provides a transcription system, which has higher translation efficiency and lower dsRNA generation compared with a conventional HEPES/Tris system, and a phosphate system can be used simultaneously with the novel All In One transcription system.

Dependencies are inferred from the claim text.