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US 2026/0028649 A1Published application
Yeast expressing a heterologous phospholipase for ethanol production
AssigneeNovozymes A/S
InventorsChee-Leong Soong, James Ron Huffman, Monica Tassone, Jung Yi, Hanlin Ouyang
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Abstract
Described herein are recombinant fermenting organisms having a heterologous polynucleotide encoding a phospholipase. Also described are processes for producing a fermentation product, such as ethanol, from starch or cellulosic-containing material with the recombinant fermenting organisms.
Claims21
1-20. (canceled)
21:
- A method of producing a fermentation product from a starch-containing or cellulosic-containing material comprising:
- (a) saccharifying the starch-containing or cellulosic-containing material;
- and
- (b) fermenting the saccharified material of step (a) with a fermenting organism;
- wherein the fermenting organism comprises a heterologous polynucleotide encoding a phospholipase.
2 more elements
22:
- The method of claim 21, wherein the phospholipase is a Phospholipase A or a Phospholipase C.
23:
- The method of claim 21, wherein the phospholipase has a mature polypeptide sequence with at least 80% sequence identity to the amino acid sequence of any one of SEQ ID NOs:
- 235-242 and 252-342.
24:
- The method of claim 21, wherein saccharification of step (a) occurs on a starch-containing material, and wherein the starch-containing material is either gelatinized or ungelatinized starch.
25:
- The method of claim 24, comprising liquefying the starch-containing material by contacting the material with an alpha-amylase prior to saccharification.
26:
- The method of claim 25, wherein liquefying the starch-containing material and/or saccharifying the starch-containing material is conducted in presence of exogenously added protease.
27:
- The method of claim 21, wherein fermentation is performed under reduced nitrogen conditions.
28:
- The method of claim 21, wherein the fermenting organism comprises a heterologous polynucleotide encoding a glucoamylase.
29:
- The method of claim 21, wherein the fermenting organism comprises a heterologous polynucleotide encoding an alpha-amylase.
30:
- The method of claim 21, wherein the fermenting organism comprises a heterologous polynucleotide encoding a protease.
31:
- The method of claim 21, wherein the fermenting organism is a Saccharomyces cerevisiae cell.
32:
- The method of claim 21, wherein the method results in higher yield of fermentation product and/or reduced foam accumulation when compared to the same process using an identical cell without the heterologous polynucleotide encoding the phospholipase under the same conditions.
33:
- The method of claim 1, wherein the method results in at least 0.25% higher yield of fermentation product.
34:
- A recombinant yeast cell comprising a heterologous polynucleotide encoding a phospholipase.
35:
- The recombinant yeast cell of claim 34, wherein the phospholipase is a Phospholipase A or a Phospholipase C.
36:
- The recombinant yeast cell of claim 34, wherein the phospholipase has a mature polypeptide sequence at least 80% sequence identity, to the amino acid sequence of any one of SEQ ID NOs:
- 235-242 and 252-342.
37:
- The recombinant yeast cell of claim 34, wherein the fermenting organism comprises a heterologous polynucleotide encoding a glucoamylase.
38:
- The recombinant yeast cell of claim 34, wherein the fermenting organism comprises a heterologous polynucleotide encoding an alpha-amylase.
39:
- The recombinant yeast cell of claim 34, wherein the fermenting organism comprises a heterologous polynucleotide encoding a protease.
40:
- The recombinant yeast of claim 34, wherein the cell is a Saccharomyces cerevisiae cell.