Back to results
US 2026/0028602 A1Published application
Split recombinases having inducible recombinase activity
AssigneeAsimov Inc.
InventorsJeremy J. Gam, Maya L. Kaul, Alec A.K. Nielsen
Life of this patent
Estimating the term…
Abstract
Described herein are split recombinases having inducible recombinase activity and polynucleic acid molecules encoding the same. Also described herein are kits and host cells comprising the split recombinases, as well as methods of their use.
Claims54
A polynucleic acid molecule encoding a polypeptide dimer having recombinase activity, wherein the nucleic acid sequence encoding the polypeptide dimer comprises, from 5′ to 3′:
- (i) a sequence encoding for a first polypeptide comprising a first portion of a recombinase and a first dimerization domain;
- (ii) a sequence encoding for a viral 2A peptide and/or an internal ribosomal entry site (IRES);
- and (iii) a sequence encoding for a second polypeptide comprising a second portion of a recombinase and a second dimerization domain;
- wherein the first polypeptide and the second polypeptide lack recombinase activity in the absence of dimerization;
- and wherein a recombinase dimer comprising the first polypeptide and the second polypeptide has recombinase activity.
2 more elements
Dependencies are inferred from the claim text.