Managing peripheral blood cells
AssigneeSino-Biocan (Shanghai) Biotech Ltd.
InventorDongbing WEI
Life of this patent
Estimating the term…
Abstract
Methods, devices, and systems for managing peripheral blood cells are provided. In one aspect, a fully automatic peripheral blood separation method includes: adding a peripheral blood sample liquid in a sample bag and a gradient liquid into a centrifuge cup, collecting a target cell liquid into an intermediate bag after centrifugation of the peripheral blood sample liquid and the gradient liquid in the centrifuge cup, adding the target cell liquid in the intermediate bag and a washing liquid to the centrifuge cup for washing and replacement to obtain a pre-product, and mixing the pre-product and a cell dilution liquid to obtain a final product.
Claims20
A fully automatic peripheral blood separation method, comprising:
- adding a peripheral blood sample liquid in a sample bag and a gradient liquid into a centrifuge cup;
- collecting a target cell liquid into an intermediate bag after centrifugation of the peripheral blood sample liquid and the gradient liquid in the centrifuge cup;
- adding the target cell liquid in the intermediate bag and a washing liquid to the centrifuge cup for washing and replacement to obtain a pre-product;
- and
- mixing the pre-product and a cell dilution liquid to obtain a final product.
A fully automatic peripheral blood separation device, comprising:
- a plurality of components comprising at least one of:
- a centrifuge configured to provide a rotational power to a centrifuge cup,
- a spin valve configured to control opening or closing of a pipe coupled to the centrifuge cup,
- a peristaltic pump configured to pump a liquid into or out of the centrifuge cup, or
- one or more scales each configured to weigh an amount of a respective liquid, and
- a controller coupled to one or more components of the plurality of components and configured to perform one or more operations for fully automatic peripheral blood separation, the one or more operations comprising:
- controlling the spin valve to open the pipe and the peristaltic pump to pump a peripheral blood sample liquid and a gradient liquid into the centrifuge cup;
- controlling the centrifuge to rotate to separate a target cell liquid from the peripheral blood sample liquid and the gradient liquid in the centrifuge cup;
- controlling to collect the target cell liquid into an intermediate bag;
- controlling to add the target cell liquid in the intermediate bag and a washing liquid to the centrifuge cup for washing and replacement to obtain a pre-product;
- and
- controlling to mix the pre-product and a cell dilution liquid to obtain a final product.
A peripheral blood mononuclear cell (PBMC) separating and packaging device, the device comprising:
- a washing liquid assembly;
- a density gradient liquid assembly;
- a sample liquid assembly;
- a waste liquid assembly;
- a preparation liquid assembly;
- a semi-finished product liquid assembly;
- a packaging assembly;
- and
- a separation cup,
- wherein the separation cup is connected to a first three-way joint via a cup inlet pipe, and the first three-way joint is further connected to a liquid inlet pipe and a collection pipe,
- wherein the washing liquid assembly, the density gradient liquid assembly, the sample liquid assembly, and the waste liquid assembly are connected to the liquid inlet pipe via a first five-way joint, and
- wherein the preparation liquid assembly, the semi-finished product liquid assembly, and the packaging assembly are connected to the collection pipe via a first four-way joint.
Dependencies are inferred from the claim text.