Laser ablation/removal and laser induced forward transfer of biological material

A system comprising:

• a first laser configured to generate a first laser beam;
• a second laser configured to generate a second laser beam;
• one or more optical elements configured to direct the first laser beam and the second laser beam through a focusing lens;
• one or more beam control elements configured to selectively transmit a selected combination of first laser beam or the second laser beam through the focusing lens;
• a first translation stage assembly adapted to support a donor substrate, wherein the donor substrate comprises:
• a back surface;
• a front surface;
• and
• a coating disposed on the front surface, wherein the coating includes a donor material;
• a second translation stage assembly adapted to support a receiver substrate;
• and
• a controller communicatively coupled to the first and second translation stage assemblies, and the one or more beam control elements, wherein the controller is configured to:
• direct the second translation stage assembly to align the receiver substrate to a focal plane of the focusing lens;
• direct at least one of the second translation stage assembly or the one or more beam control elements to at least one of ablate or remove a portion of the receiver substrate to expose a target layer of the receiver substrate;
• direct at least one of the first translation stage or the second translation stage to align the front surface of the donor substrate to be parallel to and facing the receiver substrate, wherein the coating on the donor substrate is located at the focal plane of the focusing lens;
• and
• direct at least one of first translation stage, the second translation stage, or the one or more beam control elements to irradiate the coating through the back surface of the donor substrate to transfer a portion of the donor material to the target layer of the receiver substrate.

A method comprising:

• generating a receiver substrate;
• at least one of ablating or removing a portion of the receiver substrate to expose a target layer of the receiver substrate;
• generating a donor substrate, wherein the donor substrate comprises a back surface and a front surface;
• applying a coating to the front surface, wherein the coating includes donor material;
• aligning the front surface of the donor substrate to be parallel to and facing the receiver substrate, wherein the donor material is disposed adjacent to the target layer;
• and
• irradiating the coating through the back surface of the donor substrate with one or more laser pulses produced by a second laser to transfer a portion of the donor material to the target layer;
• scanning the donor substrate through a focal point of the second laser while irradiating the donor material with the second laser to continuously provide new donor material to transfer to the receiver substrate;
• and
• scanning the receiver substrate while irradiating the donor material with the second laser to form a selected pattern of the donor material on the target layer.