Methods of making and using nanoparticles for treatment of bacterial biofilm

A method of preventing or inhibiting bacterial biofilm formation on a substrate comprising

• providing the substrate with a layer of nanoparticles, wherein the nanoparticles comprise a core comprising gelatin, and a layer of copolymer attached to the core by an acid labile bond,
• wherein bacteria contacts the layer on the substrate and the bacteria creates an environment comprising an acidic pH and a gelatinase,
• wherein the acid labile bond is broken thereby releasing the copolymer from the core causing degradation of the layer,
• wherein the gelatinase degrades the core into portions of the core causing degradation of the layer, and
• wherein degradation of the layer inhibits or prevents bacterial adhesion to the substrate and/or bacterial biofilm formation on the substrate, and
• wherein proliferation of the bacteria is inhibited or prevented.