Three-Dimensional Two Photon Miniature Microscope Brain Imaging in Freely-Behaving Animals

An implantable multi-photon optical probe comprising:

• a probe housing having a proximal end and a distal end implantable on a sample, the probe housing configured to provide excitation radiation to the sample and to collect emitted radiation from the sample;
• an input optical fiber port in a wall of the probe housing, the input optical fiber port having an input optical fiber deposed therein, with the input optical fiber configured to guide the excitation radiation into the probe housing from outside of the probe housing, through a wall of the probe housing:
• scanning optics disposed in the probe housing, the scanning optics configured to receive the excitation radiation and to (i) laterally scan the excitation radiation over a field-of-view of the optical probe, and (ii) axially scan the excitation radiation over a range of imaging depths of the optical probe;
• a scanning lens disposed in the probe housing to receive the excitation radiation from the scanning optics, the scanning lens configured to collect the excitation radiation to focus the excitation radiation;
• and
• an objective lens disposed in the probe housing to receive the excitation radiation from the scanning lens, the objective lens being disposed in the probe housing and configured to reshape and focus the excitation radiation into the sample.