Genetically encoded bioluminescent sensors
AssigneeCentral Michigan University
InventorEric PETERSEN
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Abstract
Described herein are compositions and methods for bioluminescent analyte detection. In some embodiments, a recombinant bioluminescent polypeptide sensor is disclosed comprising a luminescent signaling domain, an analyte binding domain, and one or more peptide linkers, wherein the luminescent signaling domain is oriented in relation to the analyte binding domain such that binding of an analyte to the analyte binding domain induces a conformational change in the luminescent signaling domain to generate a luminescent signal.
Claims35
A recombinant bioluminescent polypeptide sensor comprising:
- (a) a luminescent signaling domain comprising a luciferase polypeptide that is split into two luciferase polypeptide domains;
- (b) an analyte binding domain comprising one or more acetylcholine, GABA, serotonin, or glucose binding domains, or functional variants, mutants, or fragments thereof;
- and
- (c) one or more peptide linkers;
- wherein the analyte binding domain is present between the two luciferase polypeptide domains such that binding of an analyte to the analyte binding domain induces a conformational change in the luminescent signaling domain, thereby bringing the two luciferase polypeptide domains together to generate a luminescent signal.
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