Controlling contaminants during fermentation
AssigneeNewLeaf Symbiotics, Inc.
InventorsAaron Kelley, Anthony NEUMANN,
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Abstract
Methods and compositions for use in growth of pink-pigmented facultative methylotrophs (PPFM) and/or methanotroph bacteria are provided herein, wherein the fermentation comprises hop acids to inhibit growth of bacterial contaminants.
Claims28
A method for inhibiting growth of bacterial contaminants in a culture medium comprising pink pigmented facultative methylotroph (PPFM) bacteria and/or methanotroph bacteria, wherein said method comprises:
- (a) providing a sterile fermentation medium comprising nutrients suitable for growth of PPFM and/or methanotroph bacteria;
- (b) adding hop acids to the sterile fermentation medium;
- (c) adding an inoculum of PPFM and/or methanotroph cells to the fermentation medium to provide a fermentation broth;
- and (d) maintaining the fermentation broth under conditions suitable for growth of the PPFM and/or methanotroph bacteria;
- whereby the growth of bacterial contaminants is inhibited.
A fermentation broth comprising nutrients, a growing population of pink pigmented facultative methylotroph (PPFM) bacteria and/or methanotroph bacteria, and hop acids, wherein said population of PPFM and/or methanotroph bacteria is at a titer of at least 1×107 CFU per milliliter, and said hop acids comprise beta acids at a concentration of at least 7.5 ppm.
Dependencies are inferred from the claim text.