Back to results
US 2026/0016482 A1Published application
Methods for analyzing biological samples
AssigneeSeer, Inc.
InventorIman MOHTASHEMI
Life of this patent
Estimating the term…
Abstract
Described herein are methods for analyzing biological samples by liquid chromatography-tandem mass spectrometry or direct injection mass spectrometry.
Claims138
A method comprising:
- (a) contacting a plurality of biomolecules with a surface to adsorb the plurality of biomolecules on the surface;
- and
- (b) performing mass spectrometry on the plurality of biomolecules to generate a mass spectrum, wherein the mass spectrometry is (i) performed without chromatographic separation or (ii) performed using chromatographic separation with a gradient length of at most 30 minutes.
A method comprising:
- (a) contacting a plurality of biomolecules with a surface to adsorb the plurality of biomolecules on the surface;
- (b) performing mass spectrometry on the plurality of biomolecules to generate a multiplexed mass spectrum;
- and
- (c) deconvoluting the multiplexed mass spectrum to generate a plurality of deconvoluted MS signals, wherein the deconvoluting is based on a type of the surface.
1 more element
A method comprising:
- (a) incubating a biological sample with one or more surfaces to form a biomolecule corona on the one or more surfaces;
- and
- (b) performing mass spectrometry on biomolecules from the biomolecule corona to generate a mass spectrum, wherein the mass spectrometry is (i) performed without chromatographic separation or (ii) performed using chromatographic separation with a gradient length of at most 10 minutes.
A method comprising:
- (a) incubating a biological sample with a first magnetic particle to form a first protein corona;
- (b) incubating the biological sample with a second magnetic particle to form a second protein corona, wherein the first magnetic particle and the second magnetic particle have different physicochemical properties, and wherein the first magnetic particle and the second magnetic particle are incubated with separate volumes of the biological sample;
- and
- (c) performing mass spectrometry using direct infusion on proteins from the first protein corona and the second protein corona to generate a mass spectrum, wherein separate injections are performed for the first protein corona and the second protein corona.
1 more element
Dependencies are inferred from the claim text.