Nucleic Acid Analytical Method and MALDI Matrix Used for Same
AssigneeShimadzu Corporation
InventorYuko FUKUYAMA
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Abstract
Mass spectrometry is performed on a nucleic acid contained in a sample with a matrix-assisted laser desorption/ionization mass spectrometer using a mixed matrix containing 3-hydroxypicolinic acid and 2,4-dihydroxyacetophenone or a mixed matrix containing 3-hydroxypicolinic acid and 2,4,6-trihydroxyacetophenone monohydrate. This makes it possible to detect [M+H]+ or [M−H]− of the nucleic acid and fragment ions generated by dissociation of the ions with high sensitivity.
Claims11
A nucleic acid analytical method, comprising performing mass spectrometry on a nucleic acid contained in a sample with a matrix-assisted laser desorption/ionization mass spectrometer using a mixed matrix containing 3-hydroxypicolinic acid and 2,4-dihydroxyacetophenone or a mixed matrix containing 3-hydroxypicolinic acid and 2,4,6-trihydroxyacetophenone monohydrate.
A matrix for matrix-assisted laser desorption/ionization mass spectrometry of a nucleic acid, comprising 3-hydroxypicolinic acid and 2,4-dihydroxyacetophenone, or comprising 3-hydroxypicolinic acid and 2,4,6-trihydroxyacetophenone monohydrate.
Dependencies are inferred from the claim text.