Process for production of alcohols from cell lysate
OwnerKent Evans, Paul Bloom +5inventors
InventorsKent Evans, Paul Bloom, Jenifer J. Meinholz, Madison Perry, Nicholas Tumminaro, Mattias Greer, Matthew Marzo
Life of this patent
Estimating the term…
Abstract
Disclosed are systems and methods for producing renewable alcohols by fermentative processes using cell lysate or a combination of cell lysate and whole cells, as well as the cell lysate itself. The disclosure also provides processes and systems for converting renewable alcohols to transportation fuels, such as, gasoline, jet fuel, and diesel fuels.
Claims20
A method of producing renewable alcohol, the method comprising:
- a) providing a recombinant yeast cell comprising an alcohol producing metabolic pathway;
- b) mixing the recombinant yeast cell with a source of glucose to produce a first reaction mixture and fermenting the first reaction mixture to produce alcohol;
- c) recovering the alcohol;
- d) harvesting the recombinant yeast cell;
- e) disrupting the harvested recombinant yeast cell to obtain a substantially cell-free cell lysate;
- f) mixing the substantially cell-free cell lysate with a source of glucose to produce a second reaction mixture and fermenting the second reaction mixture to produce alcohol;
- and
- g) recovering the alcohol.
A method of producing renewable alcohol, the method comprising:
- a) providing a recombinant yeast cell comprising an alcohol producing metabolic pathway;
- b) disrupting the recombinant yeast cell to obtain a substantially cell-free cell lysate;
- c) mixing the substantially cell-free cell lysate with a source of glucose to produce a reaction mixture and fermenting the reaction mixture to produce alcohol;
- and
- d) recovering the alcohol.
A composition for producing isobutanol comprising a recombinant yeast cell lysate and, optionally, a buffer, wherein the recombinant yeast cell lysate comprises:
- a) an isobutanol producing metabolic pathway comprising an enzyme selected from the group consisting of acetolactate synthase (ALS), ketol-acid reductoisomerase (KARI), dihydroxyacid dehydratase (DHAD), 2-keto-acid decarboxylase (KIVD), alcohol dehydrogenase (ADH), and combinations thereof;
- and
- b) a fragment of recombinant yeast cell wall, a fragment of recombinant yeast cell membrane, or combinations thereof.
Dependencies are inferred from the claim text.