Production Method and Culture Device of Cell Tissue
AssigneesShimadzu Corporation, The University of Osaka
InventorsMasaki KANEDA, Yamato MAEDA, Masayuki OKADA, Hikaru SHIBATA, Koji NUMATA, Rintaro YAMAMOTO, Michiya MATSUSAKI
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Abstract
The production method includes: filling a soluble liquid holding material that cures under a predetermined condition between a first substrate and a second substrate; curing the filled holding material; and separating at least one cell tissue from at least one of the first substrate and the second substrate by cutting out the cured holding material from the first substrate and the second substrate.
Claims12
A production method of at least one cell tissue, comprising:
- linearly printing at least one cell between a first substrate and a second substrate in such a manner that both ends of the at least one cell are supported on the first substrate and the second substrate, respectively;
- obtaining the at least one cell tissue by culturing the printed at least one cell;
- filling a soluble liquid holding material that cures under a predetermined condition between the first substrate and the second substrate;
- curing the filled holding material;
- and
- separating the at least one cell tissue from at least one of the first substrate and the second substrate by cutting out the cured holding material from the at least one of the first substrate and the second substrate.
A bioprinting culture device for culturing at least one cell to obtain at least one cell tissue, the culture device comprising:
- a housing in which a first chamber with a first substrate for supporting the at least one cell disposed therein, a storage chamber configured to store a culture solution, and a second chamber with a second substrate for supporting the at least one cell disposed therein are provided in this order;
- a replacement unit that replaces the solution stored in the storage chamber with a soluble liquid holding material that cures under a predetermined condition;
- and
- a separation unit,
- the at least one cell being printed linearly from the first chamber toward the second chamber,
- the separation unit separating the at least one cell tissue stored in the storage chamber from at least one of the first substrate disposed in the first chamber and the second substrate disposed in the second chamber.
Dependencies are inferred from the claim text.