Crimean-congo hemorrhagic fever virus replicon particles and use thereof

A method of producing CCHF VRP, comprising:

• transfecting a host cell comprising GPC and T7 polymerase with:
• a plasmid comprising an antigenomic copy of a CCHF virus L segment operably linked to a T7 promoter;
• a plasmid comprising an antigenomic copy of a CCHF virus S segment operably linked to a T7 promoter;
• a plasmid comprising a T7 promoter operably linked to a nucleic acid molecule encoding a CCHF virus nucleoprotein;
• a plasmid comprising a T7 promoter operably linked to a nucleic acid molecule encoding a CCHF virus L protein;
• and
• culturing the cells for a period of time sufficient to produce CCHF VRP comprising a CCHF virus L genome segment, a CCHF virus S genome segment, the GPC, or a portion thereof, the nucleoprotein and the L protein, wherein the VRP comprises CCHF virus mucin like domain, GP38, Gn and Gc glycoproteins and wherein the CCHF VRP a) does not contain a CCHF virus M genome segment or b) encodes a domain of a glycoprotein precursor (GPC) but not a full-length GPC.