Antibodies that bind to natively folded myocilin
AssigneesGeorgia Tech Research Corporation, Board of Regents, The University of Texas System
InventorsRaquel L. Lieberman, Shannon E. Hill, Athena Patterson-Orazem, Jennifer A. Maynard, Ahlam Qerqez, Laura Azouz Yuan
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Abstract
Myocilin-binding agents including antibodies and antigen binding fragments thereof and fusion proteins that immunospecifically bind the coiled-coil domain of myocilin but do not bind to misfolded myocilin are provided herein. The disclosed antibodies and antigen binding fragments and fusion proteins are useful for the detection and extraction of natively folded myocilin from a sample.
Claims13
An antibody or antigen-binding fragment thereof that specifically binds to natively folded myocilin but does not bind to misfolded myocilin;
- wherein the antibody comprises:
- a HCDR1 domain having the amino acid sequence according to SEQ ID NO:12;
- a HCDR2 domain having the amino acid sequence according to SEQ ID NO:18;
- a HCDR3 domain having the amino acid sequence FVY;
- a LCDR1 domain having the amino acid sequence according to SEQ ID NO:9;
- a LCDR2 domain having the amino acid sequence according to SEQ ID NO:10;
- and
- a LCDR3 domain having the amino acid sequence according to SEQ ID NO:11.
A method of validating trabecular meshwork cell lines comprising:
- contacting the cells with an amount of the antibody or antigen binding fragment of claim
- subjecting the cells to a detection method;
- 1;
- and
- wherein the detection of signal from the myocilin antibody indicates the presence of human trabecular meshwork cells.
Dependencies are inferred from the claim text.