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US 2026/0002939 A1Published application
Methods and systems for sensitive and multiplexed analysis of biological samples using cleavable fluorphores and click chemistry
AssigneeArizona Board of Regents on behalf of Arizona State University
InventorsJia GUO, Joshua LABAER, Thai PHAM, Yi CHEN
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Abstract
Disclosed herein are compositions, systems, platforms, and kits comprising a tyramide-TCO and a tetrazine molecule linked to a detectable marker. Also disclosed are methods of using the compositions, systems, platforms, and kits to detect one or more target molecules, such as proteins or nucleic acids, in a sample.
Claims22
A system, kit, platform, or composition comprising:
- a first component comprising a tyramide conjugated to a trans-cylooctene;
- and
- a second component,
- wherein the second component has Formula (II):
- wherein R comprises a detectable marker;
- and
- wherein n is 1 to 3.
4 more elements
(canceled)
A kit comprising:
- (a) a composition comprising the compound of Formula I and Formula II;
- (b) a composition comprising 1,3,5-Triaza-7-phosphaadamantane (PTA);
- and
- (c) a composition comprising tris(2-carboxyethyl) phosphine (TCEP).
1 more element
(canceled)
A compound having Formula (II):
- wherein R comprises a detectable marker;
- and
- wherein n is 1 to 3.
(canceled)
(canceled)
A compound having Formula (III):
- wherein n is 1 to 3.
Dependencies are inferred from the claim text.