Sequential crosslinking with hydrophobic and hydrophilic chemical crosslinkers for sample preservation

A method for reducing leakage of biological components from cells during preservation and storage, the method comprising:

• i) contacting a plurality of cells with a fixative buffer comprising a poloxamine;
• ii) removing the fixative buffer from the cells;
• and
• iii) suspending the cells in a storage buffer.

A method for reducing leakage of biological components from cells during preservation and storage, the method comprising:

• i) contacting a plurality of cells with a fixative buffer;
• ii) removing the fixative buffer from the cells;
• iii) contacting the cells with a treatment buffer comprising a poloxamine;
• iv) removing the treatment buffer from the cells;
• and
• v) suspending the cells in a storage buffer.

A method for reducing leakage of biological components from cells during preservation and storage, the method comprising:

• i) contacting a plurality of cells with a fixative buffer;
• ii) removing the fixative buffer from the cells;
• and
• iii) suspending the cells in a storage buffer comprising a poloxamine.

A method for reducing leakage of biological components from cells during preservation and storage, the method comprising:

• i) contacting a plurality of cells with a fixative buffer comprising a poloxamine;
• ii) removing the fixative buffer from the cells;
• and
• iii) suspending the cells in a storage buffer comprising a poloxamine.

A method for reducing leakage of biological components from cells during preservation and storage, the method comprising:

• i) contacting a plurality of cells with a fixative buffer comprising a poloxamine;
• ii) removing the fixative buffer from the cells;
• iii) contacting the cells with a treatment buffer comprising a poloxamine;
• iv) removing the treatment buffer from the cells;
• and
• v) suspending the cells in a storage buffer.