US 2025/0376503 A2 · PROTEIN DOUBLE-SHELL NANOSTRUCTURES AND THEIR USE

Abstract

Protein double-shell nanostructures comprising apoferritin for carrying cargo proteins of interest are provided. Such nanostructures can be used to increase rigidity of a cargo protein of interest to allow structures of small and flexible proteins to be determined by cryogenic-electron microscopy (cryo-EM). Recombinant vectors for producing protein double-shell nanostructures are also provided. The nanostructures described herein may find use in various applications in research and drug discovery.

Claims33

Dependent claims

A protein double-shell nanostructure comprising:

a) an inner shell comprising a plurality of apoferritin proteins;
b) a cargo protein of interest, wherein the cargo protein is connected to the N-terminus of the apoferritin;
and
c) an outer shell comprising a tag protein, wherein the tag protein is connected to the cargo protein of interest such that the tag protein points outward from the inner shell and increases rigidity of the cargo protein of interest.

1 more element

Dependencies are inferred from the claim text.

Facts

Application

18/029,609Filed 5 Oct 2021

Priority

6 Oct 2020

Published

11 Dec 2025

Term ends

Not estimatedThe source records don’t support an estimate.

Classification9 codes

C07K 14/47
From vertebrates: from mammals
C07K 14/79
From animals; from humans: transferrins, e.g. lactoferrins, ovotransferrins
C12N 15/86
For animal cells: viral vectors
C07K 2319/24
Containing a tag with affinity for a non-protein ligand: containing a MBP -tag

Protein double-shell nanostructures and their use

Life of this patent

Protein double-shell nanostructures and their use

Life of this patent

Abstract

Claims33