Back to results
US 2025/0375520 A1Published application
Production of immune cells
AssigneeAdaptimmune Limited
InventorsGarth HAMILTON, Nail FATKHUTDINOV
Life of this patent
Estimating the term…
Loading patent…
Loading patent…
AssigneeAdaptimmune Limited
InventorsGarth HAMILTON, Nail FATKHUTDINOV
Estimating the term…
This invention relates to methods for producing immune cells expressing a therapeutic antigen receptor. An immune cell is provided that comprises a heterologous expression cassette. The heterologous expression cassette comprises (a) a coding sequence for a production TCR, (b) a constitutive promoter operably linked to the coding sequence, (c) a 5′ targeting site, and optionally (d) a 3′ targeting site. An expression construct is then introduced into the immune cell at the site of the heterologous expression cassette. The expression construct comprises a coding sequence for a therapeutic antigen receptor and the therapeutic antigen receptor is then expressed in the immune cell. Methods, reagents for use in the methods and immune cells produced by the methods are provided.
A method for producing an immune cell expressing a therapeutic antigen receptor comprising;
A method according to any one of the preceding claims wherein the immune cell is a T cell.
A method according to any one of the preceding claims wherein the heterologous expression cassette is replaced in the immune cell by a method comprising;
A method according to any one of the preceding claims wherein the targeting site is a 5′ targeting site.
A method according to any one of the preceding claims wherein the therapeutic antigen receptor is a T cell receptor (TCR).
A method according to any one of the preceding claims wherein the expression cassette comprises 5′ and 3′ targeting sites and said targeting sites comprise a nucleotide sequence from the TCRα chain constant region.
A method according to any one of the preceding claims wherein the immune cell comprising the heterologous expression cassette is produced by a method comprising
A method according to any one of the preceding claims comprising concentrating the population of immune cells expressing the therapeutic antigen receptor.
A method according to according to any one of the preceding claims comprising storing the population of immune cells expressing the therapeutic antigen receptor.
A method according to any one of the preceding claims comprising formulating the population of immune cells expressing the therapeutic antigen receptor with a pharmaceutically acceptable excipient.
An immune cell comprising a heterologous expression cassette integrated into the genome thereof,
An iPSC comprising a heterologous expression cassette integrated into the genome thereof,
Dependencies are inferred from the claim text.