Species specific proteins for wound healing and methods of use

A preparation for treatment purposes, the preparation comprising:

• a DNA sequences for MG53e generated from samples of the species including:
• B. taurus, S. scrofa, P. tigris altaiaca, F. catus, E. caballus, E. przewalskii, C. dromedarius, C. lupus familiaris, X. tropicalis and O. hannah;
• each of said samples being individually optimized for codon usage for expression in a first cell culture;
• an expression plasmid configured to impart antibiotic resistance configured to result in unadulterated cell cultures;
• said plasmid configured to be transformed into an expression vector;
• a raised second cell culture from said expression vector, said second cell culture selected from the list of cell cultures comprising:
• bacterial, yeast, and mammalian;
• collected cells of said raised cultures configured to be lysed, whereafter an aqueous phase is passed over an affinity matrix configured to isolate a recombinant protein;
• said recumbent protein comprising a C-terminal affinity tag configured for purification of said recumbent protein;
• and
• whereby said recombinant protein is purified to at least seventy percent (70%) purity.