Enzyme-decorated nanocatalysts
AssigneeCassiopea S.P.A.
InventorKeith LADEROUTE
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Abstract
Provided herein are vesicular nanocatalysts decorated on their surface with enzymes, such as for industrial use. More particularly, the nanocatalysts include outer membrane vesicles (OMVs) presenting on their surface carbonic anhydrase or a functional fragment or derivative thereof. Uses, methods of manufacture, and compositions of the nanocatalysts are also disclosed herein.
Claims30
An engineered outer membrane vesicle (OMV) comprising a heterologous enzyme presented on the outer surface of the engineered OMV.
An engineered host cell capable of producing an OMV comprising a heterologous enzyme presented on the outer surface of the OMV, wherein the engineered host cell is a photosynthetic microorganism comprising heterologous nucleic acid encoding a fusion protein comprising the heterologous enzyme operably linked to a transmembrane protein, and wherein the fusion protein is capable of being inserted into the outer membrane of the engineered host cell such that the heterologous enzyme is presented on the outer surface of the engineered host cell.
A system for the production of an engineered outer membrane vesicle (OMV) and for catalyzing the conversion of CO2 and H2O to HCO3− and H+, the system comprising:
- (a) a first reactor configured to receive a microorganism capable of producing the engineered OMV:
- (b) a receptacle configured to receive the engineered OMV produced in the first reactor, wherein the receptable is capable of being in conditions suitable for carrying out the conversion of CO2 and H2O to HCO3− and H+;
- and
- (c) a filtration interface connecting the first reactor to the receptable, wherein the engineered OMV produced in the first reactor is capable of passing through the filtration interface into the receptacle.
Dependencies are inferred from the claim text.