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AssigneeNuclera Ltd
InventorsMichal Jan Horka, Sumit Kalsi, Tobias William Barr Ost, Andreas Michael Waeber, Nikolaos Emmanouilidis
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Provided herein are methods for the controlled filling of multiple reservoirs on a microfluidic device with a defined area of fluid, the method comprising: a. providing a device with a plurality of wells connected via entry holes to a substrate bearing a plurality of electrodes; b. filling the device with an aqueous immiscible filler liquid such that the plurality of wells are at least partially filled with the filler liquid; c. loading two or more of the wells with an aqueous liquid which descends in the wells; and d. withdrawing a portion of the filler liquid from the device, thereby drawing the aqueous liquids onto the plurality of electrodes through the entry holes in order to form multiple differently sized volumes of aqueous liquid.
Disclosed is a method of loading multiple differently sized volumes of aqueous liquid into a digital microfluidic device having a substrate bearing a plurality of electrodes, the method comprising:
The method according to any one preceding claim, wherein the aqueous reagent being loaded into the device contains a DNA construct and/or reagents for cell-free protein synthesis.
The method according to any one preceding claim, wherein at least 32 volumes of aqueous liquid are formed having at least 2 different sizes.
The method according to any one preceding claim wherein the sides of the wells are shaped to form a continuous curve having no inflection points.
The method according to any one preceding claim wherein the filler fluid is withdrawn through two ports at different corners of the device using an automated syringe pump.
Dependencies are inferred from the claim text.