Ambient-stable affinity chromatography device

A method for producing an affinity chromatography device for capturing biological molecules,

• the method comprising:
• coupling a ligand to a solid support material to form a sorbent bed in an aqueous medium, wherein the ligand is configured to bind specific biological molecules;
• immobilizing the sorbent bed on or in a carrier;
• and
• drying the sorbent bed.

A method for producing an affinity chromatography device for capturing biological molecules,

• the method comprising:
• (a) coupling a ligand to a solid support material to form a sorbent bed in an aqueous medium, wherein the ligand is configured to bind specific biological molecules;
• (b) drying the sorbent bed to produce a dry powder;
• (c) reconstituting the dry powder in an aqueous medium;
• (d) immobilizing the sorbent bed on or in a carrier;
• and
• (e) drying the sorbent bed.

A method for determining the presence of a specific biological molecule in a sample, the method comprising:

• (a) reconstituting an affinity chromatography device for capturing biological molecules in an aqueous medium, wherein the device comprises:
• a carrier, and
• a sorbent bed comprising
• a solid support material;
• and
• a ligand configured to bind specific biological molecules coupled to the support material,
• wherein the sorbent bed is immobilized on or in the carrier, and
• wherein the sorbent bed is in a dry format;
• (b) contacting the sorbent bed with the sample, wherein the ligand binds specifically to the specific biological molecule when it is present;
• and
• (c) determining if the ligand bound the specific biological molecule, wherein if the ligand bound the molecule, the specific biological molecule is determined to be present in the sample.