Method and device for liquid delivery to the adsorbent layer
OwnerAneta HALKA-GRYSINSKA, Anna KLIMEK-TUREK +2inventors
InventorsAneta HALKA-GRYSINSKA, Anna KLIMEK-TUREK, Radoslaw L. GWARDA, Tadeusz H. Dzido
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OwnerAneta HALKA-GRYSINSKA, Anna KLIMEK-TUREK +2inventors
InventorsAneta HALKA-GRYSINSKA, Anna KLIMEK-TUREK, Radoslaw L. GWARDA, Tadeusz H. Dzido
Estimating the term…
To develop the chromatogram, a prepared plate is placed in a chromatographic chamber where a tip, connected to multiple supply lines for different eluent components, enters from below. Each line includes a reservoir, pump, and tubing system. The tip is moved along a set path by a three-dimensional machine while the eluents are pumped with varying flow rates, controlled by a computer to produce a changing eluent composition over time. A digital camera tracks the position of the eluent front, and this data is used by the computer to adjust pump operations in real-time. Once the eluent front reaches its final position, pumping stops. The plate is then removed and dried, resulting in a developed chromatogram.
A method of liquid delivery to an adsorbent layer of a chromatographic plate, in which method a liquid stream is directed to an outer surface of the adsorbent layer, characterized in that the liquid stream is composed of at least two individual streams (26a . . . 26x), differing in quantitative and/or qualitative composition of their components, which flow in separate tubes (8a, 9a, 9b, 9c . . . 9x, 42a, 42b, 42c), which tubes in the final part of their length are connected close to each other to allow combination of the individual streams (26a . . . 26x) that emerge from the tips of the tubes (9a . . . 9x, 42a, 42b, 42c), and the individual streams combine in the space between the tips of the tubes (9a . . . 9x, 42a, 42b, 42c) and the outer surface (28) of the adsorbent layer (24), then the combined individual streams are a collective liquid stream (32), which, using a coordinate machine (2, 43), moves repeatedly together with the tips of the tubes (9a, 9b, 9c . . . 9x, 37, 42a, 42b, 42c) along a given path over and on the outer surface (28) of the adsorbent layer (24), preferably the individual streams (26a . . . 26x) are pure solvents or a solution (mixture) of these solvents and/or solution of solid and/or liquid and/or gaseous substances in one and/or various solvents, preferably the proportion of individual streams of liquid supplied to the adsorbent layer changes continuously or gradually/in steps during the development of the chromatogram and is changed depending on the migration distance of the front of the liquid/solution developing the chromatogram or depending on the time of the chromatogram development process identical to the time of liquid delivery to the adsorbent layer.
A device for supplying liquids to the adsorbent layer (24) of a chromatographic plate (18), comprising:
Dependencies are inferred from the claim text.