Media, kits and methods for differentiating tenocytes or chondrocytes
AssigneeStemcell Technologies Canada Inc.
InventorsArthur SAMPAIO, Alessandro DEI
Life of this patent
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Abstract
Disclosed herein are media, kits, and methods for differentiating mammalian progenitor cells to a downstream lineage of cells. Embodiments of disclosed media, methods and kits may be used to differentiate mammalian progenitor cells to tenocytes or tenocyte-like cells, or chondrocytes or chondrocyte-like cells.
Claims28
A method of differentiating mammalian progenitor cells to a downstream lineage of cells, comprising culturing the mammalian progenitor cells in a basal medium comprising at least one first growth factor and optionally at least one second growth factor,
- wherein the downstream lineage of cells are tenocytes or tenocyte-like cells, or the downstream lineage of cells are chondrocytes or chondrocyte-like cells.
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A method of differentiating mammalian progenitor cells to a downstream lineage of cells, comprising:
- a) generating a fated cell population by contacting the mammalian progenitor cells with a first medium comprising a basal medium and a TGF superfamily agonist;
- and
- b) biasing the fated cell population to the downstream lineage of cells by contacting the population with a second medium comprising a basal medium and a TGF superfamily agonist.
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The method of claim 167, wherein the TGF superfamily agonist in the first medium is TGF-β1, TGF-β2, or TGF-β3, preferably TGF-β3, and the TGF superfamily agonist in the second medium is an agonist of BMP signaling, wherein the first medium does not contain the agonist of BMP signaling and the second medium does not contain the agonist of TGF signaling.
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A kit for differentiating mammalian progenitor cells to i) tenocytes or tenocyte-like cells, and/or ii) chondrocytes or chondrocyte-like cells, comprising:
- a) a basal medium;
- b) a first supplement comprising an agonist of TGF signaling;
- and
- c) a second supplement comprising an agonist of BMP signaling.
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Dependencies are inferred from the claim text.