Genetically modified human stem cell expressing a mutant human cytochrome p450 2b6 protein and its use thereof in the treatment of cancer

An isolated genetically modified human stem cell, wherein the human stem cell comprises an exogenous nucleic acid comprising a region encoding a fusion protein comprising a mutant human cytochrome P450 2B6 protein (CYP2B6*) of SEQ ID No. 1 or a variant or fragment thereof, said variant or fragment having at least 80% identity with the amino acid sequence SEQ ID No. 1 and comprising the residues 114V, 199M and 477W as shown in the amino acid sequence SEQ ID No. 1;

• and a NADPH-cytochrome P450 reductase protein of SEQ ID No. 2 or a variant or fragment thereof, said variant or fragment having at least 80% identity with the amino acid sequence SEQ ID No. 2, operably linked to a promoter, said exogenous nucleic acid having been inserted into the intron located between exon 3 and exon 4 of the ZZEF1 gene at site 4115336 on chromosome 17 of said human stem cell.