Methods for production of tissue resident memory-like t cells and use thereof
AssigneeBoard of Regents, The University of Texas System
InventorsCassian YEE, Farah HASAN
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Abstract
Provided herein are methods for the production of tissue resident memory-like T cells by the combination of hypoxia and TGFβ. Further provided herein are methods of using the tissue resident memory T cells as adoptive cell therapy.
Claims25
1-80. (canceled)
A TRM-like T cell, wherein the T cell has essentially no expression of CXCR6 protein.
A pharmaceutical composition comprising a population of TRM-like T cells with essentially no expression of CXCR6 protein and a pharmaceutically acceptable carrier.
94-109. (canceled)
A method of treating an immune-related disorder in a subject comprising administering an effective amount of TRM-like T cells produced by the method comprising:
- (a) obtaining a starting population of T cells;
- (b) culturing the starting population of T cells in hypoxic conditions or in the presence of a hypoxia-inducing agent to generate early effector cells, wherein the culturing is in media essentially free of transforming growth factor beta 1 (TGF-β1);
- and
- (c) further culturing the early effector cells in the presence of TGF-β1 to produce TRM-like T cells.
115-118. (canceled)
(canceled)
122-124. (canceled)
Dependencies are inferred from the claim text.