Metal-binding polypeptide
AssigneesEberhard Karls Universität Tübingen Medizinische Fakultät, Max-Planck-Gesellschaft zur Forderung der Wissenschaften e.V.
InventorsJulia Skokowa, Mohammad El Gamacy
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Abstract
The present invention relates to a polypeptide for use as a metal-binder, a protein comprising said polypeptide, a nucleic acid molecule encoding said polypeptide or protein, an expression vector comprising the nucleic acid molecule, a recombinant host cell comprising said polypeptide, protein, nucleic acid molecule and/or expression vector, a pharmaceutical composition comprising the said polypeptide, protein, nucleic acid molecule, expression vector and/or host cell, and to a kit.
Claims27
A polypeptide comprising an amino acid sequence having at least 60% and at most approx. 98% homology with the amino acid sequence of copper storage protein from Methylosinus trichosporium OB3b (Csp1).
The polypeptide of any of the preceding claims, which comprises any of the following amino acid sequences:
- SEQ ID NO:
- 3 (plr1), SEQ ID NO:
- 4 (plr2), SEQ ID NO:
- 5 (plr1_cr3), SEQ ID NO:
- 6 (plr1_cr61), SEQ ID NO:7 (plr1_cr62), SEQ ID NO:8 (plr1_neg1), SEQ ID NO:9 (plr1_neg2).
A protein comprising:
- a) a single polypeptide chain derived from copper storage protein from Methylosinus trichosporium OB3b (Csp1);
- b) a bundle of four amphiphatic α-helices located on said single polypeptide chain;
- c) three amino acid linkers that connect contiguous bundle-forming α-helices;
- wherein the protein comprises at least one metal binding site.
Dependencies are inferred from the claim text.