Aav5 capsid with non-canonical amino acid incorporation and uses thereof

Abstract

Aspects of the disclosure relate to compositions and methods for delivering a transgene (e.g., a transgene encoding one or more gene products) to a target cell. The disclosure is based, in part, on adeno-associated virus (AAV) capsid protein variants characterized by tropisms for certain cell types (e.g., neurons, lung cells muscle cells, bone cells, heart cells, etc.). In some embodiments, recombinant AAVs (rAAVs) comprising the capsid protein variants described herein exhibit enhanced lung tropism when compared to rAAVs having certain wild-type AAV capsid proteins (e.g., AAV5). Methods of delivering an rAAV comprising the AAV capsid protein variants are also described by the disclosure.

Dependencies are inferred from the claim text.