Menaquinone-binding compounds and methods of use thereof
AssigneeThe Rockefeller University
InventorsSean Brady, Lei Li, Bimal Koirala
Life of this patent
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Abstract
The present invention provides methods, compositions, and articles of manufacture useful for treatment of multidrug-resistant pathogens and related conditions. The present invention provides compositions and methods incorporating and utilizing menaquinone-binding compounds or derivatives or variants thereof.
Claims24
A compound comprising the amino acid sequence (XA)aG(XB)bL(XC)cW(XD)d,
- wherein each occurrence of XA, XB, XC, and XD is independently selected from the group consisting of a natural amino acid, functionalized natural amino acid, unnatural amino acid, functionalized unnatural amino acid, and any combination thereof;
- and
- wherein each occurrence of a, b, c, and d is independently an integer from 0 to 100.
An isolated nucleic acid encoding the amino acid sequence (XA)aG(XB)bL(XC)cW(XD)d or a fragment thereof,
- wherein each occurrence of XA, XB, XC, and XD is independently selected from the group consisting of a natural amino acid, functionalized natural amino acid, unnatural amino acid, functionalized unnatural amino acid, and any combination thereof;
- and
- wherein each occurrence of a, b, c, and d is independently an integer from 0 to 100.
A genetically engineered cell, wherein the cell encodes at least one amino acid sequence (XA)aG(XB)bL(XC)cW(XD)d or a fragment thereof,
- wherein each occurrence of XA, XB, XC, and XD is independently selected from the group consisting of a natural amino acid, functionalized natural amino acid, unnatural amino acid, functionalized unnatural amino acid, and any combination thereof;
- and
- wherein each occurrence of a, b, c, and d is independently an integer from 0 to 100.
A genetically engineered cell, wherein the cell comprises at least one amino acid sequence (XA)aG(XB)bL(XC)cW(XD)d or a fragment thereof,
- wherein each occurrence of XA, XB, XC, and XD is independently selected from the group consisting of a natural amino acid, functionalized natural amino acid, unnatural amino acid, functionalized unnatural amino acid, and any combination thereof;
- and
- wherein each occurrence of a, b, c, and d is independently an integer from 0 to 100.
A method of biosynthesizing a compound comprising the amino acid sequence (XA)aG(XB)bL(XC)cW(XD)d, wherein the method comprises:
- a) providing a nucleic acid to a host, wherein the nucleic acid encodes the amino acid sequence (XA)aG(XB)bL(XC)cW(XD)d or a fragment thereof;
- b) incubating the host in a growth medium;
- and
- c) isolating the compound from the host or the growth medium,
- wherein each occurrence of XA, XB, XC, and XD is independently selected from the group consisting of a natural amino acid, functionalized natural amino acid, unnatural amino acid, functionalized unnatural amino acid, and any combination thereof;
- and
- wherein each occurrence of a, b, c, and d is independently an integer from 0 to 100.
Dependencies are inferred from the claim text.